FIG. 2.

Growth and fluorescence of C. gattii and C. neoformans on m-FDTG agar. Plate 1 shows the typical growth and brown pigmentation on m-FDTG agar of C. gattii (B and C) and neoformans (A and D) after 5 to 10 days. Substituting glycine for d-proline resulted in the growth of C. neoformans, which produced light-brown pigments. Strong fluorescence was observed for the lighter-pigmented C. neoformans when it was illuminated with light of 365 nm. In contrast, the fluorescence disappeared once C. gattii produced large amounts of the brown pigment(s). Plate 3 (less typical than plate 1) shows C. gattii (C) producing a moderate amount of brown pigment which is not able to mask the fluorescence at 365 nm (plate 4).