Detection of O. viverrini DNA in human fecal samples by PCR. Ethidium bromide staining of PCR products separated in a 2% agarose gel is shown. (a) O. viverrini egg-positive specimens. Lane M is a 100-bp marker, lane P is a positive control. Lane 1, 442 EPG; lane 2, 1,120 EPG; lane 3, 180 EPG; lane N, negative control. (b) Parasite-negative specimens. Lane M, 100-bp marker; lane P, positive control. Lanes 1 to 3 contain DNAs from parasite-negative specimens. Lane N, negative control.