Abstract
The nonpolar lipids of methanol-grown bacteria which utilize one-carbon (C1) compounds via the RMP pathway (Pseudomonas C, Pseudomonas methylotropha, and Methylomonas methanolica) were found to contain squalene in concentrations between 0.1 to 1.16 mg/g of cell (dry weight). Squalene could not be detected in lipid extracts of methanol-grown bacteria which utilize C1 compounds via the serine pathway.
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Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Battat E., Goldberg I., Mateles R. I. Growth of Pseudomonas C on C1 compounds: continuoous culture. Appl Microbiol. 1974 Dec;28(6):906–911. doi: 10.1128/am.28.6.906-911.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Bird C. W., Lynch J. M., Pirt F. J., Reid W. W. Steroids and squalene in Methylococcus capsulatus grown on methane. Nature. 1971 Apr 16;230(5294):473–474. doi: 10.1038/230473a0. [DOI] [PubMed] [Google Scholar]
- Bouvier P., Rohmer M., Benveniste P., Ourisson G. Delta8(14)-steroids in the bacterium Methylococcus capsulatus. Biochem J. 1976 Nov;159(2):267–271. doi: 10.1042/bj1590267. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Cooney C. L., Levine D. W. Microbial utilization of methanol. Adv Appl Microbiol. 1972;15:337–365. doi: 10.1016/s0065-2164(08)70096-0. [DOI] [PubMed] [Google Scholar]
- De Souza N. J., Nes W. R. Sterols: isolation from a blue-green alga. Science. 1968 Oct 18;162(3851):363–363. doi: 10.1126/science.162.3851.363. [DOI] [PubMed] [Google Scholar]
- Förster H. J., Biemann K., Haigh W. G., Tattrie N. H., Colvin J. R. The structure of novel C35 pentacyclic terpenes from Acetobacter xylinum. Biochem J. 1973 Sep;135(1):133–143. doi: 10.1042/bj1350133. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Goldberg I., Jensen A. P. Phospholipid and fatty acid composition of methanol-utilizing bacteria. J Bacteriol. 1977 Apr;130(1):535–537. doi: 10.1128/jb.130.1.535-537.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Goldberg I., Rock J. S., Ben-Bassat A., Mateles R. I. Bacterial yields on methanol, methylamine, formaldehyde, and formate. Biotechnol Bioeng. 1976 Dec;18(12):1657–1668. doi: 10.1002/bit.260181202. [DOI] [PubMed] [Google Scholar]
- Haigh W. G., Förster H. J., Biemann K., Tattrie N. H., Colvin J. R. Induction of orientation of bacterial cellulose microfibrils by a novel terpenoid from Acetobacter xylinum. Biochem J. 1973 Sep;135(1):145–149. doi: 10.1042/bj1350145. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Kramer J. K., Kushwaha S. C., Kates M. Structure ditermination of the squalene, dihydrosqualene and tetrahydrosqualene in Halobacterium cutirubrum. Biochim Biophys Acta. 1972 May 23;270(1):103–110. doi: 10.1016/0005-2760(72)90183-x. [DOI] [PubMed] [Google Scholar]
- Kushwaha S. C., Pugh E. L., Kramer J. K., Kates M. Isolation and identification of dehydrosqualene and C 40 -carotenoid pigments in Halobacterium cutirubrum. Biochim Biophys Acta. 1972 Mar 23;260(3):492–506. doi: 10.1016/0005-2760(72)90064-1. [DOI] [PubMed] [Google Scholar]
- Reitz R. C., Hamilton J. G. The isolation and identification of two sterols from two species of blue-green algae. Comp Biochem Physiol. 1968 May;25(2):401–416. doi: 10.1016/0010-406x(68)90349-6. [DOI] [PubMed] [Google Scholar]
- Ribbons D. W., Harrison J. E., Wadzinski A. M. Metabolism of single carbon compounds. Annu Rev Microbiol. 1970;24:135–158. doi: 10.1146/annurev.mi.24.100170.001031. [DOI] [PubMed] [Google Scholar]
- Schubert K., Rose G., Hörhold C. Cholesterin in Streptomyces olivaceus. Biochim Biophys Acta. 1967 Feb 14;137(1):168–171. [PubMed] [Google Scholar]
- Schubert K., Rose G., Wachtel H., Hörhold C., Ikekawa N. Zum Vorkommen von Sterinen in Bakterien. Eur J Biochem. 1968 Jul;5(2):246–251. doi: 10.1111/j.1432-1033.1968.tb00364.x. [DOI] [PubMed] [Google Scholar]
- Suzue G., Tsukada K., Nakai C., Tanaka S. Presence of squalene in Staphylococcus. Arch Biochem Biophys. 1968 Mar 11;123(3):644–644. doi: 10.1016/0003-9861(68)90187-2. [DOI] [PubMed] [Google Scholar]
- Suzue G., Tsukada K., Tanaka S. Occurrence of dehydrosqualene (C30 phytoene) in Staphylococcus aureus. Biochim Biophys Acta. 1968 Sep 2;164(1):88–93. doi: 10.1016/0005-2760(68)90074-x. [DOI] [PubMed] [Google Scholar]
- TCHEN T. T., BLOCH K. On the mechanism of enzymatic cyclization of squalene. J Biol Chem. 1957 Jun;226(2):931–939. [PubMed] [Google Scholar]
- Tornabene T. G., Kates M., Gelpi E., Oro J. Occurrence of squalene, di- and tetrahydrosqualenes, and vitamin MK8 in an extremely halophilic bacterium, Halobacterium cutirubrun. J Lipid Res. 1969 May;10(3):294–303. [PubMed] [Google Scholar]
- Yamamoto S., Lin K., Bloch K. Some properties of the microsomal 2,3-oxidosqualene sterol cyclase. Proc Natl Acad Sci U S A. 1969 May;63(1):110–117. doi: 10.1073/pnas.63.1.110. [DOI] [PMC free article] [PubMed] [Google Scholar]