FIG. 2.
(A) Binding of MAbs 2F5, 13H11, and 5A9 to HIV-1 MPER peptides. Biotinylated full-length HR-2 peptide, SP62, or 8926 was immobilized at 200 to 300 RU on a streptavidin (SA) sensor chip. Biotin-DP107 was used as a control to subtract nonspecific binding. Representative overlay of specific (control subtracted) binding curves show binding of each of the MAbs to HR-2 (solid line), SP62 (dotted line), or 8926 (broken line) peptides. (B) Kd and rate constants of each of the indicated MAbs binding to HIV-1 MPER peptides. Rate constants were calculated using the bivalent analyte model to account for the avidity of the bivalent MAb. ka, association constant.