FIG. 3.

Cross-blocking of 2F5 and 13H11 MAb binding to HR-2 peptide. Biotin-HR-2 (about 200 RU) peptide was anchored on a streptavidin chip (Biacore SA chip) and either blocking antibody 13H11 (A) or 2F5 (B) was injected until the peptide surface was near saturation. Following the blocking step, either 2F5 (A) or 13H11 (B) was injected, and binding was monitored for MAb blocking. Binding of each MAb in the absence of blocking antibody (solid line) or in the presence of blocking antibody (dotted line) is shown. Arrows indicate the injection point for each MAb.