TABLE 3.
Genotypes and phenotypes of recombinant viruses
| Strain | UL97 mutation(s) | UL97 variationa | IC50 (μM)b
|
MBV IC50 ratioc | |
|---|---|---|---|---|---|
| GCV | MBV | ||||
| T2211 | None | H587Y | 1.1 ± 0.05 | 0.11 ± 0.004 | |
| T2233 | None | None | 1.1 ± 0.04 | 0.12 ± 0.003 | |
| T2241 | None | H587Y | 1.2 ± 0.08 | 0.10 ± 0.004 | |
| T2890 | V353A | None | 1.7 ± 0.1 | 1.7 ± 0.06 | 15 |
| T2942 | H411N | None | 1.1 ± 0.1 | 1.0 ± 0.05 | 9 |
| T2923 | H411L | H587Y | 0.77 ± 0.08 | 7.6 ± 0.5 | 69 |
| T2893 | H411Y | H587Y | 0.5 ± 0.02 | 1.3 ± 0.04 | 12 |
| T2903 | V353A, H411L | H587Y | 1.1 ± 0.1 | 25 ± 1.4 | 227 |
| T2960 | V353A, H411Y | H587Y | 0.67 ± 0.02 | 18 ± 0.8 | 164 |
a
Amino acid change resulting from incorporation of unique SwaI restriction site.
b
Mean ± SEM of at least 12 determinations done on three or more setup dates.
c
MBV IC50compared with that of baseline strain T2211.