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. 2007 Oct 3;82(1):555–564. doi: 10.1128/JVI.01694-06

FIG. 3.

FIG. 3.

TRAIL regulates DV replication. (A) TRAIL blocking monoclonal antibody increases DV progeny. Monocytes, B cells, HUVECs were infected with DV at an MOI of 1 PFU/cell and then cultured for 48 h. TRAIL blocking antibody (50 ng/ml) was added 24 h prior to infection with DV. DV copy number was quantified by qRT-PCR analysis. β-Actin mRNA, a constitutively expressed protein, was used as a control probe. The numbers of DV-infected cells are the means of five experiments for monocytes and two experiments each for B cells and HUVECs. Results for nonspecific antibody-treated monocytes are the means of three experiments (± SD). (B) rTRAIL treatment inhibits DV copy number. Monocytes were infected with DV at an MOI of 0.1 PFU/cell and then cultured for 24 and 48 h. Monocytes were pretreated with rTRAIL 6 h prior to infection with DV. DV copy number was quantified by qRT-PCR analysis. β-Actin mRNA, a constitutively expressed protein, was used as a control probe. Results are the means of two experiments. Mo, monocyte.