FIG. 7.

Cell death flow cytometry-based assay of CD1a⁺ cells. Absence of apoptosis in rTRAIL-treated DV-infected DCs. Monocytes were pretreated overnight with rTRAIL as described in Materials and Methods, followed by infection with DV for 48 h at an MOI of 1. Live/Dead Aqua fluorescence was used to identify apoptotic DCs at 12 (A), 24 (B), and 48 (C) h after rTRAIL treatment and DV infection. Data from a representative experiment are shown for each time point from a total of two (12 h and 24 h) and five (48 h) experiments. FITC, fluorescein isothiocyanate; APC, allophycocyanin.