TABLE 2.
qRT-PCR validation of the 11 DV response genes
| Gene name | Relative increase in induction (n-fold) determined by:a
|
|
|---|---|---|
| GeneChip analysis | PCR | |
| ISG20 | 15 | 3 |
| LYE6E | 8 | 4 |
| USP18 | 14 | 5 |
| HERC5 | 9 | 6 |
| HSXIAPAF1 | 7 | 8 |
| IF144 | 27 | 10 |
| G1P2 | 28 | 15 |
| LGALS3BP | 3 | 15 |
| IFI44L | 58 | 31 |
| IFIT1 | 126 | 32 |
| IRF7 | 12 | >100 |
a
qRT-PCR validation of the 11 DV response genes. In vitro DV infections in HUVECs were analyzed by qRT-PCR using microfluidic cards. Relative induction was calculated with qRT-PCR software (Applied Biosystems). The results of qRT-PCR and the Affymetrix GeneChip HG-U133A were consistent for HUVECs and monocytes, with 8 (>70%) of the 11 genes induced within fourfold of each other.