FIG. 5.

Incorporation of RV G and tm-RFP into virions. (A) Density gradient-purified supernatant virions were analyzed by Western blotting using the RV N, M, and G protein-specific antisera. tm-RFP was detected through a G-specific antibody which recognizes the transmembrane domain of RV G protein. The specificity of the tm-RFP signal was also tested with an antibody recognizing DsRed and its derivatives (not shown). (B) The infectious virus titer of each density gradient fraction was determined on BSR T7/5 cells and compared with the virus protein content.