FIG. 2.

Mapping a region of the inhibitory element sufficient to confer inhibition. Synthetic transcripts encoding firefly luciferase with the listed bases from the pol 5′ leader (refer to Fig. 1A for sequences) were translated in vitro in RRL. All reactions were programmed with equivalent amounts of in vitro-transcribed RNA. Following in vitro translation, samples were assayed for luciferase activity. Luc2 RNA contains a negative-control 5′ leader 87 bases in length. Results are presented as percentages of firefly luciferase activity relative to the luc2 control levels from three independent experiments, except the results for pol15-28, which were from two experiments, with error bars representing the standard errors of the means.