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. 2007 Oct 24;82(1):77–85. doi: 10.1128/JVI.01484-07

FIG. 6.

FIG. 6.

Regulation of Pol translation during HSV-1 infection. (A) Western blot analyses of Pol (top panels) and ICP8 (bottom panels) from Vero cells infected with either polΔ54.x (left panels), a mutant virus with the pol inhibitory element deleted, or wt.x (right panels), a control virus with the wild-type pol sequence, for the times indicated. Mock-infected Vero cell lysate was also run (lane 2). (B) Northern blot analysis of pol (top) and ICP8 (bottom) transcript levels during a time course of infection with either polΔ54.x or wt.x. (C) Quantification of the Western blot analyses performed by running a dilution series for both Pol and ICP8 to generate a standard curve that was used to determine the concentrations of Pol and ICP8 at 6 and 9 hpi in Vero cells infected with either wt.x (black bar) or polΔ54.x (gray bar) at an MOI of 10. Protein levels were also adjusted for differences in transcript levels from panel B. Values are presented as changes (n-fold) in Pol levels observed after normalization to ICP8 levels and adjustment for mRNA levels relative to the value for wt.x at each time point.