FIG. 2.

Induction of IFN-α/β in CEFs infected with different influenza viruses. (A) CEFs were infected with the indicated influenza viruses, and the UV-treated supernatants were used to examine IFN-mediated inhibition of VSV-GFP replication in CEFs as described in Materials and Methods. The cells were visualized by fluorescence microscopy at 12 and 24 h p.i. (B) RT-PCR analysis of IFN-α/β- and chicken β-actin-specific mRNAs from CEFs infected with R-SW/FJ/03 (lane 1), SW/FJ/01-03NS (lane 2), R-SW/FJ/01 (lane 3), or SW/FJ/03-01NS (lane 4) or from mock-infected CEFs (lane 5). Lane 6, marker.