TABLE 1.
Primers used in this study
| Purpose | Primer sequence (5′-3′)a
|
|
|---|---|---|
| Forward | Reverse | |
| PB2 amplification | CCAGCAAAAGCAGGTCAAATATATTC | TTAGTAGAAACAAGGTCGTTTTTAAACAAT |
| PB1 amplification | CCAGCAAAAGCAGGCAAACCATT | TTAGTAGAAACAAGGCATTTTTTCAT |
| PA amplification | CCAGCAAAAGCAGGTACTGATCCAAAAT | TTAGTAGAAACAAGGTACTTTTTTGGAC |
| HA amplification | CCAGCAAAAGCAGGGGTCTAATTTGCC | TTAGTAGAAACAAGGGTGTTTTTAACTAC |
| NP amplification | CCAGCAAAAGCAGGGTAGATAATCACTC | TTAGTAGAAACAAGGGTATTTTTCTTTA |
| NA amplification | CCAGCAAAAGCAGGAGTTCAAAATGAATC | TTAGTAGAAACAAGGAGTTTTTTGAACA |
| M amplification | CCAGCAAAAGCAGGTAGATGTTGAAAG | TTAGTAGAAACAAGGTAGTTTTTTAC |
| NS amplification | CCAGCAAAAGCAGGGTGACAAAAAC | TTAGTAGAAACAAGGGTGTTTTTTATC |
| NS1 expression | GCCGCCACCATGGATTCCAACACTGTGTCAAGC | GCTATCAAACTTCTGACTCAATTGTTCTC |
| NS2 splice acceptor site mutation | ATGAGGATGTGAAAAATGCAATTGG | CCAATTGCATTTTTCACATCCTCAT |
| NS1 HA tag introduction | GCCACCATGACCAGCTACCCATACGATGTTCCAGATTACGCTGCAGATTCCAACACTGTGTCAAGC | TTAGTAGAAACAAGGGTGTTTTTTATC |
| Chicken CPSF amplification | GCCACCATGCAGGAGCTCAT | CTTTCACTGTCCACTGAG |
| CPSF Flag tag introduction | GCTGCCACCATGCAGGAGCTCATCG | GACTTACTACTTGTCATCGTCATCCTTGTAGTCGATGTCATGATCTTTATAATCACCGTCATGGTCATGGTCTTTGTAGTCAGCCTGTCCACTGAGAAAGGCCAG |
| Chicken IFN-α amplification | GGGTACGACATCCTGTTGCTC | CGGCTGATCCGGTTGAGGAG |
| Chicken IFN-β amplification | GCCACAGCCTCCTCAACCAGAT | CAACGTCCCAGGTACAAGCACT |
| Chicken β-actin amplification | AGACAGCTACGTTGGTGATGAAGCCCAG | GTTGAAGGTAGTTTCATGGATACCACAG |
a
Nucleotides that have been changed are shown in italics.