TABLE 4.
Disease and death caused by H5N1 viruses in chickens after i.v. inoculationa
| Virus | Titer (log EID50/ml) | No. of chickens with manifestation
|
IVPI | |
|---|---|---|---|---|
| S/D/totalb | SC/totalc | |||
| SW/FJ/01 | 7.7 | 0/10/10 | / | 2.5 |
| SW/FJ/03 | 8.0 | 10/0/10 | 10/10 | 0.3 |
| R-SW/FJ/01 | 8.0 | 0/10/10 | / | 2.2 |
| R-SW/FJ/03 | 8.3 | 10/0/10 | 10/10 | 0.2 |
| SW/FJ/01-03PB2 | 8.3 | 0/10/10 | / | 2.1 |
| SW/FJ/01-03HA | 8.3 | 0/10/10 | / | 2.5 |
| SW/FJ/01-03M | 8.0 | 0/10/10 | / | 1.9 |
| SW/FJ/01-03NS | 8.3 | 4/0/10 | 10/10 | 0.2 |
| SW/FJ/03-01PB2 | 7.8 | 10/0/10 | 10/10 | 0.2 |
| SW/FJ/03-01HA | 8.3 | 10/0/10 | 10/10 | 0.3 |
| SW/FJ/03-01M | 7.8 | 10/0/10 | 10/10 | 0.2 |
| SW/FJ/03-01NS | 8.0 | 0/10/10 | / | 1.9 |
Six-week-old White Leghorn chickens housed in high-efficiency particulate air-filtered isolators were inoculated i.v. with 0.2 ml of a 1:10 dilution of bacterium-free allantoic fluid containing virus for IVPI testing, based on the Office International Des Epizooties recommendations.
S/D/total, numbers of chickens that were sick (S) and died (D) as well as total number of chickens during the observation period. Birds that showed disease signs, such as depression and ruffled feathers, but recovered at the end of the observation were counted as sick animals.
SC/total, number of chickens that seroconverted/total number of chickens at the end of the observation period. /, all birds died by the end of the observation period.