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ps20 upregulates CD54. Cells (2 × 105) (ps20low EV versus ps20hi G91 Jurkat cells or ps20+ clone 86 1-3 versus ps20− NP clone 86 1-1) were directly stained for CD11a (a and c, respectively) and CD54 (b and d, respectively) by standard direct immunostaining, and MFIs from replicate cultures were determined. Cells were precultured with either 5 μg/ml control mouse IgG or IG7 for 4 days prior to staining. Untreated controls were included as indicated.
