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. 2007 Oct 3;82(1):487–494. doi: 10.1128/JVI.01875-07

FIG. 1.

FIG. 1.

Colinear schematic diagram of DNA fragments used to map a novel lethal temperature-sensitive mutation in the ts8-22 viral genome. (Line 1) Diagram of the HSV-1 genome and XbaI E fragment. The open rectangles represent repeated sequences flanking the ends of unique long (UL) or unique short (US) sequences. The approximate site of the XbaI E fragment in the UL segment is indicated. (Line 2) Expanded diagram of the XbaI E fragment showing XbaI/BglII fragments screened for the ability to rescue the temperature-sensitive phenotype. The fragments are designated 1 and 2 as shown and in Table 1. (Line 3) Expanded fragment 1, as shown in line 2, illustrating BamHI sites. Fragment 1 contains the entire BamHI D, H, and H′ fragments and truncated C′ and O fragments. Truncations of the last two fragments are indicated by brackets. (Line 4) Expansion of the truncated BamHI C′ fragment. The position of a NarI site used to divide the fragment into fragments 3 and 4 is shown. B, BamHI; Bg, BglII; N, NarI; X, XbaI.