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. 2007 Oct 17;82(1):546–554. doi: 10.1128/JVI.01689-07

FIG. 1.

FIG. 1.

Specificity of an Adv-specific CTL line for patient 11. Adv-specific CTL lines were screened by an ELISPOT assay against 11 pools of hexon peptides (20-mer overlapping by 15 aa) to identify which peptide pools were being recognized by each CTL line. (A) The CTL line generated for patient 11 showed specific IFN-γ release against peptide pools 4 and 9. Results are expressed as numbers of spot-forming cells (SFC)/1 × 105 CTL. (B) To identify the stimulating 20-mer peptides, the CTL line for patient 11 was rescreened against the individual peptides contained in pools 4 and 9. The CTL reactivity against pool 4 mapped to the overlapping peptides 12 (SRELMGQQSMPNRPNYIAFR) and 13 (GQQSMPNRPNYIAFRDNFIG), while the reactivity in pool 9 mapped to peptides 4 and 5. Results are expressed as numbers of SFC/2 × 105 CTL.