FIG. 3.
Representative cell line functional avidity. (A) Two thousand Tat28-35SL8-specific CD8+ T cells were mixed with 10,000 autologous B-LCLs in each well and stimulated with wild-type Tat28-35SL8 peptide (crosses) or one of four variant peptides, S1P (squares), T2I (triangles), S5L (diamonds), or L8P (circles). Each peptide was titrated over a 7-log range of concentrations, from 10 μM down to 10 pM, and assayed in duplicate. The average number of spots per well is shown, with error bars representing the standard deviation. (B) Two hundred thousand Nef165-173IW9-specific CD8+ T cells were stimulated with 100,000 autologous B-LCLs pulsed with the wild-type Nef IW9 (crosses), I1T (circles), I1A (squares), and I1T+T6M (diamonds) peptides titrated over a 7-log range of concentrations, from 10 μM down to 10 pM. The percentage of CD8+ lymphocytes secreting IFN-γ and/or TNF-α in response to each peptide is shown.