FIG. 4.

Median delta HTL responses induced after INX102-3697 DNA construct immunization. H-2^bxd mice were immunized once i.m. with 100 μg of pDNA after pretreatment with cardiotoxin. Direct ex vivo IFN-γ ELISPOT results are expressed as median delta SFC/10⁶ CD4⁺ cells from four pools of CD4⁺ cells, each from five mice, with one standard deviation. In addition, the number of pools showing a positive response (≥30 SFC/10⁶ CD8⁺ cells and a response ratio of ≥2) is indicated below the x axis. An epitope is considered immunogenic if a positive response is detected in at least one pool of mice.