Skip to main content
. 2007 Oct 24;82(1):268–277. doi: 10.1128/JVI.01588-07

FIG. 3.

FIG. 3.

The d3-4 ICP27 polypeptide is unable to promote cytoplasmic localization of ICP0 and ICP4 in transfected cells. Vero cells were transfected with ICP0-expressing (A and B) or ICP4-expressing (D and E) plasmids, with or without plasmids encoding either WT ICP27 or d3-4 ICP27. Cells were fixed 1 day later and processed for ICP0 or ICP4 immunofluorescence. Localization patterns of ICP0 and ICP4 were scored as predominantly nuclear (N), predominantly cytoplasmic (C), or mixed (N = C, N>C, or C>N). Representative staining patterns for ICP0 and ICP4 are shown in panels A and D, respectively. The data in panels B and E represent the mean values from two independent experiments. (C and F) Immunoblot analysis of ICP0 and ICP4 in transfected cells. Vero cells were transfected as for panels A, B, D, and E, and accumulation of ICP0, ICP4, and ICP27 at 1 day posttransfection was analyzed by immunoblotting. As a loading control, the levels of cellular protein EEA1 were also determined.