FIG. 5.

Analysis of purified WT HSV-1 and d3-4 virions. (A and B) Immunoblotting analysis. Extracellular virions were prepared as described in Materials and Methods, using either one (A) or two (B) rounds of 5 to 15% Ficoll gradient purification. For comparison, total protein extracts from the same infected cells are also shown. Virions and cell lysates were analyzed by immunoblotting using antibodies specific for the viral proteins listed. In panel A, the results from two separate virion preparations are shown. For ICP0 and ICP4, asterisks mark the positions of the major 110-kDa and 175-kDa species, respectively. (C) Protein profiles. Equivalent amounts of the twice-gradient-purified virions used for panel B were separated by SDS-PAGE and visualized by silver staining. The positions of molecular weight standards are shown, as is the band corresponding to major capsid protein VP5 (arrow).