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. 2007 Nov 28;82(3):1505–1517. doi: 10.1128/JVI.01331-07

FIG. 8.

FIG. 8.

Infection of caveolin-1-deficient Huh7 cells with pseudotypes of LCMV and LFV. (A) Detection of caveolin-1 in total lysates of Huh7 and HeLa cells by Western blotting. The loading control was the detection of α-tubulin. (B) Infection of Vero E6 and Huh7 cells with VSV pseudotypes of LFV and LCMV. Cells were infected with 200 infectious units of the indicated VSV-based pseudotypes, and the infection status was determined after 24 h by the detection of the GFP reporter. Doublets of infected cells were counted as one infectious event (means ± standard deviations; n = 3). (C) Infection of Huh7 cells with LCMV and LFV pseudotypes is dependent on membrane cholesterol. Huh7 cells either were treated with 10 mM MBCD for 1 h (cholesterol depletion) or were incubated for 16 h with nystatin/progesterone (cholesterol sequestration) and infected with 200 infectious units of the indicated VSV pseudotypes. Infection was assessed by the detection of the GFP reporter as described for panel B (values are means ± standard deviations; n = 3).