FIG. 3.

LANA2 protein interacts with tubulin. (a) In vitro-translated [³⁵S]methionine LANA2 protein was incubated with purified tubulin and subjected to an in vitro polymerization assay. Assembled microtubules were separated from unpolymerized tubulin by centrifugation. Shown are LANA2 and tubulin detected in supernatants (S [unpolymerized tubulin]) and pellets (P [polymerized tubulin]). (b) In vitro-translated [³⁵S]methionine LANA2 deletion constructs were incubated with purified tubulin and subjected to an in vitro polymerization assay. Shown are LANA2 deletion constructs and tubulin in supernatants (S) and pellets (P). (c) LANA2 colocalizes with tubulin in KSHV-infected BCP-1 cells. Cytospins of BCP-1 cells were double-stained with anti LANA2 and anti-alpha-tubulin antibody. The colocalization of both proteins is shown in yellow. Areas A, B, C, and D in panel b correspond to areas designated for bp 1 to 1706.