FIG. 5.

Cotransfection of pcDNA-LANA2 and Bac36 DNA decreases the microtubule polymerization induced by paclitaxel. HEK293 cells were transfected with Bac36 DNA, together with empty pcDNA or pcDNA-LANA2, and then treated with 10 μM paclitaxel for 24 h. Cells were then fixed and stained with anti-LANA2 and anti-alpha-tubulin antibodies. Cells expressing GFP, that is, those containing Bac36, and not expressing LANA2 show aster-like microtubule changes or altered tubulin structures induced by paclitaxel treatment, while those positive for GFP (green), and thus carrying Bac36, together with LANA2 (red), did not show the effects of paclitaxel.