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. 2007 Nov 21;82(3):1581–1590. doi: 10.1128/JVI.01375-07

FIG. 6.

FIG. 6.

Effect of calpain inhibition on EV1 RNA replication. (A) The proportion of infected cells, following EV1 RNA microinjection, in the presence or absence of calpeptin (140 μM) in SAOS-α2β1 cells 5 h p.i. The numbers of negative-strand (B) and genomic-strand (C) EV1 RNA copies analyzed by quantitative RT-PCR in the presence or absence of calpeptin (140 μM) at the indicated time points p.i. are shown. Negative-strand (D) and positive-strand (E) EV1 RNA copies in the presence or absence of calpain inhibitors 1 and 2 (130 μM) at the indicated time points p.i. also are shown. (F) The number of genomic-strand (positive) EV1 RNA copies analyzed by quantitative RT-PCR in the presence or absence of calpeptin (Calp; 140 μM) and protease inhibitors antipain (Ant; 500 μM), leupeptin (Leu; 500 μM), aprotinin (Apr; 5 U/ml), trypsin inhibitor (Try; 2 mM), and elastatinal (Ela; 250 μM) 6 h p.i. (G) The effect of calpeptin on in vitro cell-free translation of EV1 RNA in the rabbit reticulocyte system. The control (C) sample does not contain EV1 RNA.