FIG. 4.

Survival of CG-4 cells expressing agnoprotein. (A) CG-4 cells expressing JCV agnoprotein and control agnonegative cells were plated equally in triplicate in two sets. One set of each cell line was induced to differentiate into oligodendrocytes, and cell viability was evaluated by MTT assay. The percentage of induced cells relative to that for uninduced was determined for each cell line at the third day of induction. (B) Genomic DNA was prepared from agnopositive and agnonegative CG-4 cells induced to differentiate into oligodendrocytes for 6 days and analyzed on a 1.2% agarose gel. (C) Comet nucleus formation in progenitor CG-4 cells was analyzed by alkaline comet assay. Adenoviral vectors expressing JCV agnoprotein (Ad-Agno) and control adenovirus vector without a transgene (Ad-null) were used for transduction of CG-4 progenitor cells. From two experimental sets, one set was induced to differentiate into oligodendrocytes after infection/transduction and the second set was left as an uninduced control. Single-cell gel electrophoresis was performed at the third day following induction. The average OTM was scored randomly for 100 cells for each condition by use of Comet software.