FIG. 5.

Expression of agnoprotein sensitizes CG-4 progenitors differentiating into oligodendrocytes to H₂O₂. CG-4 progenitors, expressing GFP-agnoprotein and agnonegative (GFP only), were plated in two sets, and one of them was induced to differentiate into an oligodendrocyte lineage. At the fourth day of induction, cells were harvested and half of the cells from each sample was treated with H₂O₂. (A) The comet assay was performed, and images were classified in three groups according to the extent of DNA damage as follows: OTM of <6, no damage; OTM of 6 to 25, moderate damage; OTM of >25, extensive damage. (B) The percentages of cells with different levels of DNA damage for each sample are presented in bars.