FIG. 6.

Detection of cleaved caspase-3 in agnopositive cells upon induction as oligodendrocytes. (A) Cell lysates prepared from agnopositive (GFP-agnoprotein) and agnonegative (GFP only) cells at the third and sixth days following the induction of differentiation were subjected to immunoblot analysis for the detection of uncleaved and cleaved caspase-3. Equal loading was verified with anti-α-tubulin antibody. (B) Caspase-3/7 activity was measured for cell lysates prepared from agnopositive (GFP-agnoprotein) and agnonegative (GFP-only) cells at the fourth and sixth days following the induction of differentiation and for uninduced controls.