FIG. 7.

Effect of mutant agnoprotein on apoptosis of undifferentiated and differentiated CG-4 cells. (A and B) Cell cycle distribution and apoptosis (Apo) of CG-4 cell lines expressing mutant agnoprotein were analyzed by fluorocytometry. (C) DNA-laddering assay from cells expressing wild-type or mutant agnoprotein (GFP-agnoprotein*). (D) DNA fragmentation upon differentiation of cells expressing wild-type agnoprotein (GFP-agno) or mutant agnoprotein (GFP-agno*) was analyzed by comet assay. The measured average OTMs are presented.