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. 2007 Nov 28;82(3):1195–1203. doi: 10.1128/JVI.01692-07

FIG. 3.

FIG. 3.

Capsid residues determining MVM tropism in culture. (A) Selection of forward mutants in A9 mouse fibroblasts from genetically independent stocks derived by transfection with infectious clones. The sequences of the assayed LB2 and LB3 mutants are shadowed, with residues changed by directed mutagenesis in bold. (B) Interaction of MVMi mutants with mouse hemopoietic cells. Nonadherent myeloid cells harvested from long-term bone marrow cultures were prestimulated for 3 days in IMDM-10% FCS-10% WEHI-3B conditioned medium as a source of interleukin-3 and infected with the indicated viruses at a multiplicity of infection of 5 PFU/cell, and the number of viable cells was scored daily. (C) Susceptibility of the BFU-E mouse hemopoietic precursors to MVMi mutants. Total C57BL or BALB/c bone marrow cells were infected at the indicated multiplicity of infection and cultured in vitro under conditions allowing the growth of this committed erythroid precursor.