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. 2007 Nov 28;82(3):1570–1580. doi: 10.1128/JVI.01673-07

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FIG. 2. — The α_Vβ₃ integrin mediates cell adhesion to KSHV gB(RGD). (A) Fluorescence-activated cell sorter analysis of integrin levels in the pBJ parental CHO cells compared to the levels in β₃-CHO and α₃-CHO recombinant cells expressing chimeric human-hamster α_Vβ₃ and α₃β₁ integrins, respectively, using anti-α_Vβ₃ (LM609) and anti-α₃ (ASC-6) integrin antibodies. (B) Fluorescence-activated cell sorter analysis of α_Vβ₃ levels in pBJ-CHO, β₃-CHO, and α₃-CHO cells. (C to F) Cell adhesion to immobilized gB(RGD) peptide (10 μg/ml) by β₃-CHO (C), α₃-CHO (D), and the parental pBJ-CHO (E) cells and to immobilized laminin by α₃-CHO cells (F) is shown. Bar, 20 μm. (G) Cell adhesion to increasing concentrations of gB(RGD) peptide. The data represent the mean numbers ± standard errors of adherent cells in quadruplicate wells. Control experiments showed no adhesion to the gB(RGE) peptide with a single-amino-acid mutation in the RGD domain (data not shown).