FIG. 3.
The RGD motif of KSHV gB specifically binds the αVβ3 integrin. Magnetic beads coated with the KSHV gB(RGD) peptide (lanes 2, 5) or gB(RGE) control peptide (lanes 3, 6) were incubated with lysates from either the β3-CHO (lanes 2, 3) or α3-CHO (lanes 5, 6) cells. Bound proteins were eluted with EDTA and characterized by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and immunoblot analysis using anti-β3 antibody (MAb 2023) to detect αVβ3 (lanes 1 to 3) and anti-α3 antibody (MAb 2290) to detect α3β1 (lanes 4 to 6). Integrin levels in the initial β3-CHO (lane 1) and α3-CHO (lane 4) cell lysates are shown.