FIG. 4.

α_Vβ₃ function-blocking antibodies inhibit gB(RGD)-mediated HT1080 cell adhesion. (A) Flow cytometry analysis of cell surface integrin levels on HT1080 human fibrosarcoma cells using anti-α₃ (ASC-6), anti-α_Vβ₅ (P1F6), anti-α₅β₁ (JBS5), or anti-α_Vβ₃ (LM609) antibodies. (B) HT1080 cell adhesion to immobilized gB(RGD) peptide (10 μg/ml) in the presence of different concentrations of the function-blocking integrin antibodies used for the experiments whose results are shown in panel A. Adherent cells were quantitated manually. The data represent the mean cell numbers ± standard errors of the results for triplicate wells. Standard errors smaller than the plot symbol are not shown.