TABLE 1.

Results of analysis of mutations in the context of the GIT and S2204I-bearing replicon

Constructa	GIT replicationb	GIT colony size	S2204I replicationb	S2204I colony size	Western blot	Real-time PCR (in GIT)
GIT	1.6 × 106-2.18 × 106	Normal	NAc	NA	Normal	Replication
S2204I	NA	NA	3.64 × 106	Normal	NDd	ND
C3-4 (M313A)	23	Normal	1.20 × 104	Small	Normal	No replication
D1-1 (P314A)	0		0		Defect	No replication
D1-3 (W316A)	1.43 × 103	Small	20	Small	Normal	No replication
D2-1 (A317G)	1.60 × 104	Small	1.00 × 104	Small	Normal	ND
D3-2 (Y321A)	25	Small	20	Small	Normal	No replication
E1-2 (P324A)	0		6.75 × 105	Small	Defect	No replication
E3-1 (W329A)	0		0		Normal	No replication
F1-1 (V338A)	0		0		Normal	No replication
F2-1 (G341A)	0		10	Normal	Normal	No replication
F2-2 (C342A)	0		0		Normal	No replication
F2-3 (P343A)	0		0		Defect	No replication
F3-1 (L344A)	10	Normal	10	Normal	Normal	No replication

^aClone designations used in manuscript (single-letter amino acid code of the wild-type residue, amino acid position in the NS5A protein, and the amino acid residue present in the mutant).

^bReplication as determined by G418 transduction assay (in CFU per microgram of input RNA).

^cNA, not applicable.

^dND, not determined.