FIG. 1.

Effects of lysosomotropic weak bases on PCV2 Stoon-1010 infection of PK-15 cells. PK-15 cells were treated with RPMI 1640 (control), 25 mM NH₄Cl, or 125 μM CQ from 1 h before virus inoculation until 24 h after virus inoculation. The cells were inoculated with the same dose of PCV2 (Stoon-1010) for 1 h. At 36 h after virus inoculation, the cells were fixed and stained for PCV2 antigens by IPMA. Magnification, ×50.