FIG. 3.

Effects of CQ at different time points and durations throughout the PCV2 infection cycle. PK-15 cells were inoculated with the same dose of PCV2 Stoon-1010 for 1 h (0 to 1 h p.i.), after which the viral inoculum was washed off. CQ (0 or 125 μM) was added at different times before (b.i.) or after (p.i.) virus inoculation and left with the cells for different durations, as indicated. After 36 h p.i., the cells were fixed and stained for PCV2 antigens. The percentages of PCV2-infected PK-15 cells following CQ treatment are expressed relative to the number of PCV2-infected PK-15 cells among untreated cells. The data represent means plus standard deviations of results from three experiments, with each experimental condition performed in quadruplicate.