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. 2007 Nov 21;82(3):1146–1154. doi: 10.1128/JVI.01698-07

TABLE 2.

Replication of transfectant viruses in micea

Virus Mean virus titer (log10 EID50/ml ± SD) at indicated day p.i. in:
MLD50 (log EID50)
Lung
Spleen
Kidney
Brain
Day 4 p.i. Day 6 p.i. Day 4 p.i. Day 6 p.i. Day 4 p.i. Day 6 p.i. Day 4 p.i. Day 6 p.i.
DK/12 3.9 ± 0.5 3.6 ± 0.7 < < < < < < 6.4
DK/27 7.3 ± 0.1 6.9 ± 0.4 3.6 ± 0.3 2.0 ± 0.9 2.9 ± 0.9 3.0 ± 0.1 2.2 ± 0.8 3.1 ± 0.1 0.6
R-DK/12 3.5 ± 0.1 3.5 ± 0.6 < < < < < < 6.4
R-DK/27 7.2 ± 0.3 6.9 ± 0.4 3.8 ± 0.3 1.8 ± 0.6 2.7 ± 0.7 2.9 ± 0.1 2.0 ± 0.5 2.8 ± 0.3 0.6
DK/12-27PB2 4.5 ± 0.5 4.5 ± 0.7 < < < < < < 6.4
DK/12-27PA 5.9 ± 0.4c 4.1 ± 1.0 < < < < < < 6.4
DK/12-27HA 3.8 ± 0.3 4.5 ± 0.4 < < < < < < 6.4
DK/12-27NP 5.1 ± 0.3c 5.5 ± 0.5b < < < < < < 6.4
DK/12-27NS 5.8 ± 0.6c 6.9 ± 0.4c 2.3 ± 0.1 2.6 ± 0.3 1.6 ± 0.3 1.6 ± 0.5 < 3.1 ± 0.3 2.0
DK/27-12PB2 5.6 ± 0.5e 5.9 ± 0.5 2.2 ± 0.8e 2.8 ± 0.4 2.0 ± 0.5 2.9 ± 0.5 1.5 ± 0.1 2.9 ± 0.5 0.6
DK/27-12PA 5.7 ± 0.4e 6.4 ± 0.1 2.1 ± 0.6d 2.3 ± 0.8 1.3 ± 0.1d 1.9 ± 0.4d 1.9 ± 0.7 2.5 ± 0.4 1.5
DK/27-12HA 6.8 ± 0.3 5.8 ± 0.5d 3.5 ± 0.7 3.0 ± 0.5d 2.4 ± 0.1 2.5 ± 0.4 1.4 ± 0.1 2.4 ± 0.9 1.5
DK/27-12NP 6.5 ± 0.4 6.3 ± 0.6 3.1 ± 0.6 2.5 ± 0.4 2.4 ± 0.1 2.2 ± 0.1 1.4 ± 0.1 2.9 ± 0.4 0.6
DK/27-12NS 3.9 ± 0.5e 4.9 ± 0.5e < < < < < < 6.4
DK/12NS1P42S 6.5 ± 0.4c 6.8 ± 0.3c 2.0 ± 0.7 2.0 ± 0.7 1.3 ± 0.1 1.3 ± 0.1 < 3.3 ± 0.8 2.2
DK/12NS1N48S 3.4 ± 0.1 4.5 ± 0.4 < < < < < < 6.4
DK/27NS1S42P 4.5 ± 0.4e 5.8 ± 0.3d < < < < < < 6.4
DK/27NS1S48N 7.3 ± 0.1 7.5 ± 0.1 2.5 ± 1.1 2.8 ± 0.8 1.7 ± 0.7 3.1 ± 0.8 2.2 ± 0.9 3.1 ± 0.3 0.8
DK/27NS1R38A 7.6 ± 0.3 7.3 ± 0.8 2.1 ± 0.8 1.4 ± 0.1 2.1 ± 0.6 2.1 ± 0.6 1.4 ± 0.1 2.4 ± 0.1 0.8
DK/27NS1K41A 6.6 ± 0.3 6.5 ± 0.4 1.7 ± 0.4 1.4 ± 0.1 1.6 ± 0.3 2.2 ± 0.3 1.6 ± 0.5 2.9 ± 0.4 0.6
DK/27NS1R38AK41A 5.0 ± 0.5d 4.6 ± 0.7e < < < < < < 6.4
a

Six-week-old SPF BALB/c mice were inoculated intranasally with 106 EID50 of each virus in a 50-μl volume. Three mice from each group were killed on days 4 and 6 p.i., and virus titers were determined in samples of lung, spleen, kidney, and brain in eggs. <, no virus was isolated from the sample.

b

P value was <0.05 compared with the titers in the corresponding organs of the DK/12- or R-DK/12-inoculated mice.

c

P value was <0.01 compared with the titers in the corresponding organs of the DK/12- or R-DK/12-inoculated mice.

d

P value was <0.05 compared with the titers in the corresponding organs of the DK/27- or R-DK/27-inoculated mice.

e

P value was <0.01 compared with the titers in the corresponding organs of the DK/27- or R-DK/27-inoculated mice.