TABLE 2.
Virus | Mean virus titer (log10 EID50/ml ± SD) at indicated day p.i. in:
|
MLD50 (log EID50) | |||||||
---|---|---|---|---|---|---|---|---|---|
Lung
|
Spleen
|
Kidney
|
Brain
|
||||||
Day 4 p.i. | Day 6 p.i. | Day 4 p.i. | Day 6 p.i. | Day 4 p.i. | Day 6 p.i. | Day 4 p.i. | Day 6 p.i. | ||
DK/12 | 3.9 ± 0.5 | 3.6 ± 0.7 | < | < | < | < | < | < | 6.4 |
DK/27 | 7.3 ± 0.1 | 6.9 ± 0.4 | 3.6 ± 0.3 | 2.0 ± 0.9 | 2.9 ± 0.9 | 3.0 ± 0.1 | 2.2 ± 0.8 | 3.1 ± 0.1 | 0.6 |
R-DK/12 | 3.5 ± 0.1 | 3.5 ± 0.6 | < | < | < | < | < | < | 6.4 |
R-DK/27 | 7.2 ± 0.3 | 6.9 ± 0.4 | 3.8 ± 0.3 | 1.8 ± 0.6 | 2.7 ± 0.7 | 2.9 ± 0.1 | 2.0 ± 0.5 | 2.8 ± 0.3 | 0.6 |
DK/12-27PB2 | 4.5 ± 0.5 | 4.5 ± 0.7 | < | < | < | < | < | < | 6.4 |
DK/12-27PA | 5.9 ± 0.4c | 4.1 ± 1.0 | < | < | < | < | < | < | 6.4 |
DK/12-27HA | 3.8 ± 0.3 | 4.5 ± 0.4 | < | < | < | < | < | < | 6.4 |
DK/12-27NP | 5.1 ± 0.3c | 5.5 ± 0.5b | < | < | < | < | < | < | 6.4 |
DK/12-27NS | 5.8 ± 0.6c | 6.9 ± 0.4c | 2.3 ± 0.1 | 2.6 ± 0.3 | 1.6 ± 0.3 | 1.6 ± 0.5 | < | 3.1 ± 0.3 | 2.0 |
DK/27-12PB2 | 5.6 ± 0.5e | 5.9 ± 0.5 | 2.2 ± 0.8e | 2.8 ± 0.4 | 2.0 ± 0.5 | 2.9 ± 0.5 | 1.5 ± 0.1 | 2.9 ± 0.5 | 0.6 |
DK/27-12PA | 5.7 ± 0.4e | 6.4 ± 0.1 | 2.1 ± 0.6d | 2.3 ± 0.8 | 1.3 ± 0.1d | 1.9 ± 0.4d | 1.9 ± 0.7 | 2.5 ± 0.4 | 1.5 |
DK/27-12HA | 6.8 ± 0.3 | 5.8 ± 0.5d | 3.5 ± 0.7 | 3.0 ± 0.5d | 2.4 ± 0.1 | 2.5 ± 0.4 | 1.4 ± 0.1 | 2.4 ± 0.9 | 1.5 |
DK/27-12NP | 6.5 ± 0.4 | 6.3 ± 0.6 | 3.1 ± 0.6 | 2.5 ± 0.4 | 2.4 ± 0.1 | 2.2 ± 0.1 | 1.4 ± 0.1 | 2.9 ± 0.4 | 0.6 |
DK/27-12NS | 3.9 ± 0.5e | 4.9 ± 0.5e | < | < | < | < | < | < | 6.4 |
DK/12NS1P42S | 6.5 ± 0.4c | 6.8 ± 0.3c | 2.0 ± 0.7 | 2.0 ± 0.7 | 1.3 ± 0.1 | 1.3 ± 0.1 | < | 3.3 ± 0.8 | 2.2 |
DK/12NS1N48S | 3.4 ± 0.1 | 4.5 ± 0.4 | < | < | < | < | < | < | 6.4 |
DK/27NS1S42P | 4.5 ± 0.4e | 5.8 ± 0.3d | < | < | < | < | < | < | 6.4 |
DK/27NS1S48N | 7.3 ± 0.1 | 7.5 ± 0.1 | 2.5 ± 1.1 | 2.8 ± 0.8 | 1.7 ± 0.7 | 3.1 ± 0.8 | 2.2 ± 0.9 | 3.1 ± 0.3 | 0.8 |
DK/27NS1R38A | 7.6 ± 0.3 | 7.3 ± 0.8 | 2.1 ± 0.8 | 1.4 ± 0.1 | 2.1 ± 0.6 | 2.1 ± 0.6 | 1.4 ± 0.1 | 2.4 ± 0.1 | 0.8 |
DK/27NS1K41A | 6.6 ± 0.3 | 6.5 ± 0.4 | 1.7 ± 0.4 | 1.4 ± 0.1 | 1.6 ± 0.3 | 2.2 ± 0.3 | 1.6 ± 0.5 | 2.9 ± 0.4 | 0.6 |
DK/27NS1R38AK41A | 5.0 ± 0.5d | 4.6 ± 0.7e | < | < | < | < | < | < | 6.4 |
Six-week-old SPF BALB/c mice were inoculated intranasally with 106 EID50 of each virus in a 50-μl volume. Three mice from each group were killed on days 4 and 6 p.i., and virus titers were determined in samples of lung, spleen, kidney, and brain in eggs. <, no virus was isolated from the sample.
P value was <0.05 compared with the titers in the corresponding organs of the DK/12- or R-DK/12-inoculated mice.
P value was <0.01 compared with the titers in the corresponding organs of the DK/12- or R-DK/12-inoculated mice.
P value was <0.05 compared with the titers in the corresponding organs of the DK/27- or R-DK/27-inoculated mice.
P value was <0.01 compared with the titers in the corresponding organs of the DK/27- or R-DK/27-inoculated mice.