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. 2007 Nov 28;82(3):1605–1609. doi: 10.1128/JVI.01876-07

FIG. 1.

FIG. 1.

Cloning of the canine PolI promoter. (A) Molecular map of the canine ribosomal DNA. Head-to-tail repeats of rRNA genes (18, 5.8, and 28S rRNA) are separated by IGS containing the PolI promoter and terminator regions. The PolI promoter region is located directly upstream of the 5′ ETS, and the terminator region is located downstream of the 3′ ETS. The transcription initiation site is indicated as +1. This figure is adapted from reference 18 with permission of the publisher. (B) Alignment of the PolI transcription start regions (nt −8 to +11), as predicted by computer analysis, of the canine (underlined) and other species (adapted from reference 11). The transcription initiation site is indicated as +1. (C) Sequences of the canine PolI promoter regions, as predicted by computer analysis. The region (nt −457 to +1) was cloned from the genomic DNA of MDCK cells by using specific primers indicated by the arrows (solid lines for nt −457 to +1 and broken lines for nt −250 to +1). The cloned sequence was aligned with the canine genomic DNA sequence (GenBank accession no. NW_878945).