Table 1.

Folding rates of P RNA and CP RNA during transcription at 37°C

Ribozyme	Folding process	Folding rate (s−1) by the cleavage of
		tRNA substrate	Selected substrate
P RNA	Refolding*	(5.2  ± 0.4) × 10−3	(7.8  ± 0.8) × 10−3
	T7 RNa polymerase	(5.5  ± 0.2) × 10−3	(20  ± 3) × 10−3
CP RNA	Refolding*	0.075  ± 0.013†	>0.2
		(5.3  ± 0.4) × 10−3‡	ND§
	T7 RNA polymerase	(4.8  ± 0.2) × 10−3	(23  ± 2) × 10−3
	E coli RNA polymerase
	Wild type: core	(6.8  ± 0.4) × 10−3	(23  ± 2) × 10−3
	Wild type: (+) NusA	(23  ± 2) × 10−3	(23  ± 2) × 10−3
	Mutant: core	(3.6  ± 0.2) × 10−3	ND
	Mutant: (+) NusA	(3.6  ± 0.2) × 10−3	ND

ND, not determined. 

^*Data from ref. 5. 

^†Fast-folding population. 

^‡Slow-folding population. 

^§Not determined at 37°C, but identical to that from cleaving a tRNA substrate at 28°C.