FIG. 2.

Time courses of PKR and eIF-2α phosphorylation following infection of PKR⁺, PKR^kd, and PKR^kd-con cells with ΔE3L mutant compared to WT vaccinia virus. Whole-cell extracts were prepared from uninfected cells (0) or infected cells at 3, 6, 9, and 12 h p.i. with either WT or ΔE3L virus. Whole-cell extract protein (30 μg) was analyzed in each lane. The membranes were probed with antibodies against PKR, phospho-T446-PKR, eIF-2α, phospho-eIF-2α, vaccinia virus E3L, and β-actin as a loading control.