FIG. 3.

Time course of viral gene expression following infection of PKR⁺, PKR^kd, and PKR^kd-con cells with WT compared to ΔE3L mutant vaccinia virus. Whole-cell extracts were prepared at increasing times (h) after infection with either WT or ΔE3L vaccinia virus. Ten micrograms of protein was analyzed in each lane. The membranes were probed with antibodies against the vaccinia virus early gene product I3, the early and intermediate gene product D5, and the late gene product B5. β-Actin was included as a loading control.