FIG. 5.

Vaccinia virus-mediated apoptosis is impaired in PKR-deficient HeLa cells. (A) Western immunoblot measurement of PARP cleavage with WT or ΔE3L mutant vaccinia virus at 24 h p.i. Whole-cell extract protein (10 μg) was analyzed in each lane. (B) Caspase 3/7 activation assay. Apoptosis induction by vaccinia virus infection of PKR⁺, PKR^kd, and PKR^kd-con cells was measured in 96-well plates, either mock infected or infected with the indicated deletion mutant virus at an MOI of 5. Apoptosis was measured using a Caspase-Glo 3/7 kit at 24 h p.i. Each experiment was repeated a minimum of three times. The error bars indicate standard deviations.