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Restriction enzyme digestion and Southern blot analyses. (A) pOka-BAC and pOka-BACΔgM were digested with BamHI and separated on a 0.8% agarose gel. Molecular size markers are shown at the left. (B through E) Southern blot analyses of BamHI-digested pOka-BACΔgM DNA and pOka-BAC DNA (B and C) and of rpOkaΔgM and rpOka viral DNA (D and E) were performed. The blots were treated with labeled probes for the Kmr gene (B and D) and the gM and ORF62 genes (C and E).
