FIG. 4.

Effect of EVG-selected mutations on IN strand transfer activity and on the inhibition of strand transfer by IN inhibitors. The strand transfer activities of recombinant IN enzymes carrying EVG-selected mutations were determined using an oligonucleotide-based strand transfer assay. Strand transfer (ST) activity of IN mutants was compared to that of the wild type (WT); results are shown as percentages of wild-type activity. The effect of IN inhibitors on strand transfer was also determined for wild-type and mutant IN enzymes; results are expressed as the increase (n-fold) in IC₅₀ values of inhibitors relative to those of the wild type.