FIG. 2.
Development of vaccinia virus antibodies in hyperimmunized rabbits. Two groups of rabbits were inoculated with MVA or WR to generate hyperimmune sera, as shown in Fig. 1A. (A) Serum antibody titers of individual rabbits inoculated with MVA or WR were determined by whole-virus ELISA. Shown are average titers (± SD) of three rabbits in each group. (B) Neutralization titers were determined by incubation of twofold serial dilutions of sera with a recombinant vaccinia virus that expresses enhanced GFP and then quantifying infected cells by flow cytometry. Shown are average (Avg) neutralization titers (± SD) of three rabbits in each group. IC50, 50% inhibitory concentration.