TABLE 3.
Identification of tumor risk groups and viral analysis of tumors for SV40 viruses in hamsters
| Risk levela | Virus | No. of tumors (%)b | Virus load
|
Regulatory regionc
|
||||||
|---|---|---|---|---|---|---|---|---|---|---|
| No. of tumors tested | Median no. of SV40 genome copies/tumor cell (range) | No. of tumors tested | PCR band
|
Sequence analysis
|
||||||
| WT | R | No. tested | WT | R | ||||||
| High | Baylor(1E) | 10 (83) | 10 | 4.3 (0.3-524.6) | 10 | 10 | 1 | 2 | 2 | 1 |
| SVCPC | 18 (54) | 16 | 4.2 (0.0-23.1) | 15 | 15 | 4 | 4 | |||
| CPC-PML | 11 (58) | 11 | 5.4 (0.7-24.5) | 11 | 11 | 1 | 1 | 1 | 1 | |
| Intermediate | CPC-Baylor | 7 (37) | 7 | 6.9 (1.2-18.0) | 7 | 7 | 1 | 1 | 1 | 1 |
| CPC-776 | 6 (35) | 6 | 4.2 (0.0-17.5) | 4 | 4 | 1 | 1 | |||
| CPC-VA | 4 (24) | 4 | 5.8 (0.0-48.6) | 3 | 3 | 0 | ||||
| 777 | 5 (26) | 5 | 6.3 (0.1-11.9) | 5 | 5 | 1 | 1 | |||
| 776(1E) | 5 (31) | 5 | 6.3 (0.4-10.7) | 5 | 4 | 1 | 2 | 1 | 1 | |
| 776-Baylor | 5 (33) | 4 | 16.0 (9.8-19.7) | 4 | 4 | 0 | ||||
| VA45-54(2E) | 3 (23) | 3 | 14.7 (2.7-25.3) | 3 | 3 | 1 | 1 | |||
| Low | Baylor(2E) | 1 (6) | 1 | 4.0 | 1 | 1 | 1 | 1 | ||
| 776(2E) | 3 (10) | 3 | 1.8 (1.0-4.6) | 3 | 3 | 1 | 1 | |||
| 776-CPC | 1 (7) | 1 | 0.8 | 1 | 1 | 1 | 1 | |||
| 776-VA | 4 (13) | 2 | 2.5 (1.9-3.0) | 1 | 1 | 0 | ||||
| 776-PML | 1 (6) | 0 | ||||||||
| SVPML | 0 (0) | 0 | ||||||||
| Total | 84 | 78 | 5.4 | 73 | 72 | 4 | 16 | 15 | 4 | |
a
Risk levels were defined as follows: high, >50% tumors following i.p. inoculation of weanling Syrian golden hamsters with 1 × 107 PFU of virus; intermediate, 20 to 40% tumors; low, <20% tumors.
b
Data from Table 1.
c
Abbreviations: WT, wild type (same as virus inoculated); R, rearranged regulatory region (based on gel migration of PCR product and sequence analysis).