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. 2007 Oct 31;82(2):683–691. doi: 10.1128/JVI.02049-07

FIG. 4.

FIG. 4.

Rescue of budding for NES mutant Gag proteins coexpressed with wild-type Gag. (A) Autoradiogram of a typical budding rescue assay (see Materials and Methods). Gag-GFP can be differentiated from Gag-CFP by its faster migration during electrophoresis. Note the increase in the amount of Gag in the medium for L219A.Gag-CFP when coexpressed with wild-type Gag.GFP. (B) PhosphorImager quantification of budding efficiency. Particle release for wild-type Gag-GFP was arbitrarily set to 100%, and each coexpressed CFP protein was compared to Gag-GFP. Error bars represent standard errors of the means. (C) Analysis of budding efficiency for untagged NES GagΔPR mutants expressed with wild-type Gag-GFP resulted in an increase in NES mutant budding.