FIG. 5.

Affibody-mediated viral gene transfer to Her2-expressing cells. Her2-positive 293/Her2 cells (black bars) or Her2-negative 293 cells (white bars) were transduced with Ad vectors containing either wt fiber (AdTL) or the affibody-targeted fiber chimera AdTL.11F.Her2:7. The efficacy of transduction was gauged by the activity of luciferase expressed by the viruses. Shown below the graph are the concentrations of free affibody (in μg/ml) used to inhibit cell transduction. Error bars indicate the standard deviations obtained in triplicate samples. This competitor protein had no effect on the transduction of either cell line by AdTL (not shown).