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. 2007 Nov 7;82(2):630–637. doi: 10.1128/JVI.01896-07

FIG. 6.

FIG. 6.

Transduction of human breast cancer cells with the Her2-targeted Ad vectors. The following viruses were used: wt, AdTL; lanes 1, AdTL.11F.Her2:4; lanes 2, AdTL.11F.Her2:7; lanes 3, AdTL.FF.Her2:4; lanes 4, AdTL.FF.Her2:7; lane 5, Ad5Luc1, Ad expressing only luciferase with the wt fiber; and lane 6, no virus. All transductions were done at a multiplicity of infection of 100 virus particles per cell. In all three panels, error bars indicate the standard deviations obtained in triplicate samples. (A) Comparison of gene delivery to Her2-positive SK-BR-3 and Her2-negative MDA-MB-231 cells. Each cell line was infected with either Ad vector containing wt fiber (AdTL) or Ad vector containing a Her2-targeted fiber chimera (AdTL.11F.Her2:4 or AdTL.11F.Her2:7). The efficacy of transduction was measured using the activity of vector-encoded luciferase reporter. (B) Comparison of gene delivery to SK-BR-3 cells with AdTL, AdTL.11F.Her2:4, AdTL.11F.Her2:7, AdTL.FF.Her2:4, and AdTL.FF.Her2:7. Black bars show luciferase expression by unblocked vectors. White bars show the levels of bioluminescence in the cells preincubated with a free competitor (Ad5 fiber knob for AdTL or Her2:4 affibody for targeted Ads, each used at a final concentration of 30 μg/ml). (C) Targeting of Ad with redesigned fiber-fibritin-affibody proteins yields more efficient killing of tumor cells. SK-BR-3 cells transduced with Ad vectors were treated with ganciclovir to engage the drug-converting activity of the TL transgene. The number of viable cells determined using an MTT [3-(4,5-dimethylthiazol-2-yl)2 2,5-diphenyl tetrazolium bromide] assay is shown. rlu, relative light units.